Optical sectioning using single-plane-illumination Raman imaging

Journal of Raman Spectroscopy – Volume 41, Issue 10, October 2010, Pages 1099–1101

Ishan Barman, Khay Ming Tan, Gajendra Pratap Singh

Confocal Raman imaging is widely used for optical sectioning of materials. However, for biological applications it often suffers from poor axial resolution, photodamage to the sample of interest, substrate interference, and long acquisition times. We have applied the principles of light sheet microscopy to Raman imaging and show for the first time that optically sectioned Raman images can be obtained in significantly lower acquisition times.